I B.Sc. Biotechnology student performing Microbiology experiment, streaking the bacterial culture in Laminar air flow (LAF) chamber. The chamber is equipped with HEPA (High-Efficiency Particulate Air) filters that remove 99.97{3d57c7fb7a29dafc062656b231d04045dfa4e384161ab7893a9d9c4afd59efdd} of particles > 0.3 μm. Laminar Air flow chamber provides a sterile environment to culturing the microorganisms.I B.Sc. Biotechnology students are observing the stages of Mitosis using Allium cepa root test. Allium cepa root test is applied in toxicity testing of chemicals.II B.Sc. Biotechnology students are measuring the hydrogen-ion activity in different types of buffers to determine the acidity or alkalinity (expressed as pH) with the help of pH meter.II B.Sc. Biotechnology students performing Glucose estimation assay and calculation of Glucose concentration in unknown samples. II M.Sc. Biotechnology student is placing the passaged cell culture flask in CO2 incubator to maintain an optimal environment for cell growth, by providing carbon dioxide control in a humidified atmosphere with constant temperature.II M.Sc. Biotechnology students are loading the samples in microtiter plates for performing MTT assay. MTT assay is a standard high throughput cell biology assay to determine cell viability and estimate cell metabolism.III B.Sc. Biotechnology student loading the DNA sample for target gene amplification via Polymerase Chain Reaction (PCR) in the Thermocycler. Further, the amplified product will be confirmed by agarose gel electrophoresis.III B.Sc. Biotechnology students are working on High Speed Ultra Centrifuge for pelleting of cellular organelles such as mitochondria, Microsomes, Ribosomes using gradient separation techniques
The Department of Biotechnology is equipped with state-of-the-art independent laboratories for microbiology, immunology, bioprocess technology, molecular biology, genetic engineering, plant tissue culture and animal cell culture labs for teaching and research activities. Unit operations such as media preparation room, bioinformatics lab facilities, smart class and instrumentation labs also available. The Department has several modern equipment for Biotechnology research, some of the major equipment include:
PCR (Polymerase Chain reaction)
ELISA microplate reader
UV-Visible
Spectrophotometers
High Resolution light microscope
Gel documentation system
Colorimeters
Inverted microscope
Dissection microscope
Lyophilizer
Microtome
Cryocan
Microbiological Incubators
CO2 Incubator
Fermentor (Batch and Continuous)
Ultra centrifuge
Homogenizer
Deep freezers (-20ºC)
Refrigerated centrifuges
UV-Transilluminator
Laminar airflow chambers
Bio-safety cabinet
Shaker incubator
Hot air oven
Electrophoresis systems (Western blotting and Southern blotting)
